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. 2014 Oct 13;9(10):e109082. doi: 10.1371/journal.pone.0109082

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© 2014 Heaslip et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Super-resolution STORM images of the actin cytoskeleton in INS-1 cells in low (A) and high (B) glucose conditions and after actin depolymerization with cytochalasin D (C). In each STORM image (A–C) yellow highlighted area is magnified 3x in the upper right image with skeletonized image below used for quantification of actin filament length and number of actin filament intersections in D. (D) Quantification of actin organization. Left: The length of actin per µm² of imaged cell area under various conditions. Right: The number of actin intersections per µm² of imaged cell area under various conditions. ** indicates p<0.001. Values are mean±SEM.