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. 2004 May 12;101(21):8138–8143. doi: 10.1073/pnas.0305683101

Fig. 1.

Fig. 1.

IL-1Ra is expressed by human islets and down-regulated in type 2 diabetes. Double immunostaining for IL-1Ra in green (A and C) or red (E) and insulin in red (B and D) or CD68 in green (F) in tissue sections of pancreases from a nondiabetic patient without (A, B, E, and F) and from a patient with type 2 diabetes (C and D). In situ hybridization for IL-1Ra mRNA in red (G) double immunostained for insulin in green (H) in tissue sections of pancreas from a nondiabetic patient. (I) Electron microscopy of secretory granules within a β cell in cultured human islets double gold-immunolabeled for insulin (small particles) and IL-1Ra (large particles). (J) RT-PCR analysis of IL-1Ra expression by purified human β cells (lanes 1 and 2), cultured human islets (lanes 3 and 4), human blood monocytes (lanes 5 and 6), and water (negative control; lane 7). One of four experiments from four donors is shown. Double immunostaining for IL-1Ra in red (K) and insulin in green (L) in purified human β cells.