Table 2. H. seropedicae EPS is required for biofilm formation on glass fiber.

Strains	Biofilm in glass fiber (O.D.550nm)	Biofilm in glass fiber+wild-type EPS (O.D.550nm)
H. seropedicae SmR1	0.66±0.02 a	0.67±0.02 a
H. seropedicae EPSEB	0.30±0.01 b	0.54±0.03 c

H. seropedicae strains were grown in the presence of glass fiber and purified wild type EPS (100 µg.mL⁻¹) when indicated. After 12 hours, bacteria attached to the fiber were stained with crystal violet, washed and de-stained with absolute ethanol. The absorbance of the ethanol (550 nm) was determined and subtracted from the absorbance of the control without bacteria. Different letters indicate significant difference (p<0.001, Duncan multiple range test) between biofilm formation by the strains.