Table 1. Enzyme-linked immunosorbent assay of BDNF secreted by Cop-1-reactive T cells.

Antigen	—	Cop-1	MBP	OVA	Con A
pg/ml	400 ± 45	1220 ± 100	1500 ± 150	440 ± 50	1100 ± 120

Cop-1-reactive T cells were cultured for 48 h with the indicated antigens in stimulation medium. T cell supernatants were collected and subjected to sandwich ELISA (see Supportng Text, which is published as supporting information on the PNAS web site). Compared with unstimulated T cells, secretion of BDNF by Cop-1-reactive T cells was significantly increased after stimulation of the T cells with a specific antigen (Cop-1) or with a self-antigen (MBP) that crossreacts with Cop-1. Values are mean values (pg/ml) ± SE (from three independent experiments) of the amounts of BDNF secreted by Cop-1-specific T cells in response to stimulation by various antigens. OVA, ovalbumin.