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. 2014 Jul 25;33(18):2020–2039. doi: 10.15252/embj.201489282

Figure 2. NSun2-targeted tRNAs accumulate as 5′ tRNA fragments.

Figure 2

  1. A, B Frequency of m5C in all tRNAs in NSun2-expressing (control) and NSun2-lacking (−/−) human (A) and mouse (B) tRNAs. Grey boxes indicate cytosines 48 and 49.
  2. C Detection of NSun2-binding to cytosines in tRNAs using miCLIP method. Grey box indicates cytosines 48 and 49.
  3. D Scheme of 3′ half of a tRNA cloverleaf structure showing frequency of methylation at C48, 49 (red).
  4. E Scheme of cleaved tRNA through the anticodon loop.
  5. F Average fraction of 5′ (grey box) and 3′/CCA-tagged tRNA fragments using all expressed tRNAs with (orange line) and without (blue line) a cytosine at positions 48 and 49 in NSun2-expressing mouse (upper panel) and human (lower panel) samples.
  6. G Isotype-specific enrichment of 5′ tRNA fragments (grey box) in NSun2−/− (orange line) versus respective control (blue line) in mouse (upper panel) and human (lower panel) samples.

See also Supplementary Figs S2 and S3.