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. 2014 Aug 1;33(18):2069–2079. doi: 10.15252/embj.201488698

Figure 2. PP2A associates with BAK1 in planta.

Figure 2

  1. PP2A chemical inhibition triggers BIK1 activation. BIK1 phosphorylation status was detected by anti-HA immunoblot in BIK1pro:BIK1-HA seedlings treated with mock, 100 nM flg22, or 50 μM cantharidin for the indicated time. Coomassie Brilliant Blue staining of the membrane (CBB) is shown to assess equal protein loading.
  2. Cantharidin-triggered oxidative burst is impaired in mutants of PRR complex components. ROS production in response to 100 nM flg22 or 50 μM cantharidin was measured in Col-0, fls2, bak1-4, bik1 pbl1, and rbohD. Values are average of three biological repeats ± SE presented as percentage of Col-0 response. Values labeled with different letters (regular and italic for flg22 and cantharidin treatment respectively) are statistically different as established by a one-way ANOVA (P < 0.05).
  3. PP2A activity is constitutively associated with BAK1. PP2A activity in un-elicited BIK1pro:BIK1-HA seedlings was measured by colorimetry on protein extracts enriched with anti-FLS2, anti-BAK1, or anti-HA antibodies. PP2A activity relative to background detected in non-enriched protein extracts in absence of antibodies (control) is presented as average of three biological repeats ± SE.

Source data are available online for this figure.