. Author manuscript; available in PMC: 2015 Oct 1.

Published in final edited form as: J Steroid Biochem Mol Biol. 2014 Aug 17;144PB:286–293. doi: 10.1016/j.jsbmb.2014.08.009

Table 1.

Analysis of 20(OH)D3 metabolites produced by mouse liver microsomes by mass spectrometry. Mass spectra were recorded as described in the Experimental section. Parent ions are highlighted in bold.

Product	[M + Na]⁺	[M + H]⁺	[M + H - H₂O]⁺	[M + H - 2H₂O]⁺	[M + K]⁺	Classification
A	439.3	-	399.3	381.3	455.2	Dihydroxyvitamin D3
B	439.3	-	399.3	381.3	-	Dihydroxyvitamin D3
C1	437.2	415.2	-	-	453.1	Dehydro-dihydroxyvitamin D3
C2	439.3	-	399.3	-	455.2	Dihydroxyvitamin D3
D	437.3	415.2	397.2	379.2	-	Dehydro-dihydroxyvitamin D3
E	437.1	415.2	-	-	453.1	Dehydro-dihydroxyvitamin D3