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. 2014 Oct 13;207(1):139–158. doi: 10.1083/jcb.201402079

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© 2014 Dadgar et al.

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Figure 7. — Immunostaining of inflammatory proteins CD11b, CD74, and CD163 shows expression specific to intervening areas of muscle between injuries spaced 4 d apart. (A–C) Shown are immunofluorescence stains for inflammatory protein markers CD11b (A), CD74 (B), and CD163 (C). Each panel shows matched phase contrast (marking injury sites with tattoo dyes), antigen with nuclear stains (DAPI), and antigen alone. All three inflammatory markers show preferential localization in the area between the two injury sites only when injuries are spaced 4 d apart and not 0 or 10 d apart (n = 3 muscles per group). Arrows indicate immunoreactive material in the microenvironment between two neighboring sites of regeneration.