Table 4.
Genes | P-value/FC | |||||||
27–time point single regeneration series | Double injection LCM series | |||||||
4 d between injections | 10 d between injections | |||||||
3/4 da | 9/10 da | 13/14 da | 30/40 da | Single siteb | In-betweenb | Single siteb | In-betweenb | |
ACTA2 93100_at | 0.001 | 0.006 | 0.002 | NS | NS | 0.005/8.3 | NS | 0.008/16.8 |
COL6A1 95493_at | 0.003 | 0.0001 | 0.0001 | 0.02 | NS | 0.002/−3.6 | NS | 0.00004/7.2 |
COL18A1 101039_at | 0.00001 | 0.0003 | 0.01 | NS | NS | NS | NS | 0.0001/4.0 |
COL4A2 101039_at | NS | 0.01 | 0.008 | NS | NS | NS | NS | 0.0007/4.5 |
ELN 92207_at | NS | 0.004 | 0.02 | NS | NS | NS | NS | 0.00004/3.2 |
MMP14 160118_at | 0.0007 | 0.0002 | 0.006 | NS | NS | NS | NS | 0.0006/10.6 |
COL14A1 99476_at | 0.007 | 0.004 | 0.002 | NS | NS | NS | NS | 0.0003/1.5 |
COL15A1 99637_at | 0.02 | NS | 0.006 | NS | NS | NS | NS | 0.004/1.6 |
C2 103673_at | 0.02 | 0.02 | NS | NS | NS | NS | NS | 0.000009/2.6 |
STAT3 99099_at | 0.02 | 0.002 | 0.05 | NS | NS | NS | NS | 0.003/4.4 |
CDKNA1 94881_at | 0.03 | 0.05 | 0.009 | NS | NS | NS | NS | 0.00007/3.25 |
RAMP2 994444_at | NS | 0.007 | 0.006 | NS | NS | NS | NS | 0.0007/3.5 |
ACVR2B 994444_at | NS | 0.007 | 0.006 | NS | NS | NS | NS | 0.0004/6.45 |
The 13 gene transcripts shown were significantly up-regulated in the region between asynchronous injection sites in the double LCM series when injections were separated by 10 d, but not 4 d (0/13). In the 27–time point synchronous regeneration time series, these same transcripts showed the highest and consistent up-regulated between 9 and14 d. FC, fold change.
Days are versus 0.
Sites are versus nonregenerating.