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. 2004 May 12;101(21):8221–8226. doi: 10.1073/pnas.0400459101

Fig. 2.

Fig. 2.

Transcomplementation of ΔF508-CFTR and N-tail processing mutants by an amino fragment (amino acids 1–633) of wild-type CFTR. (A and B) Coexpression of 1–633 with ΔF508-CFTR (A) or with Δ2–79-CFTR (B) in vacciniainfected COS-7 cells resulted in the appearance of band C. (C) Fragment 1–633 induced the modest appearance of band C when coexpressed with Δ2–79-CFTR in HEK-293T cells. (D) Introduction of the ΔF508 mutation in the 1–633 fragment (1–633ΔF) eliminated transcomplementation of ΔF508-CFTR in COS-7 cells but not transcomplementation of Δ2–79-CFTR. (E) The 1–633 fragment coimmunoprecipitates with ΔF508-CFTR. (F) CoIP of the band B forms of wild-type CFTR and ΔF508-CFTR with V5-tagged-1–633 from COS-7 lysates 24 h after transfection. All results are representative of 3–10 experiments.