Table 4.
Metastasis-associated biological functions of selected target transcripts of miRNA-5p and -3p sister pairs 1
| miRNA family | miRNA | Chrom’l location | Expression | Selected target mRNAs 2 | Functions 3 |
|---|---|---|---|---|---|
| let-7 | let-7 g-5p | 3p21.1 | Up-reg’d | IGF1R* | Angiogenesis, apoptosis |
| MYC* | Angiogenesis, apoptosis, cancer, Jak-STAT, MAPK & Wnt signaling | ||||
| let-7 g-3p | Up-reg’d | ZEB1 | EMT, transcriptional misregulation in cancer | ||
| NLK | Angiogenesis, MAPK & Wnt signaling | ||||
| let-7d-5p | 9q22.32 | Up-reg’d | IGF1R* | Angiogenesis, apoptosis | |
| THBS1* | Angiogenesis, cell cycle, p53 signaling, cancer | ||||
| let-7d-3p | Down-reg’d | KRAS | Apoptosis, MAPK & VEGF signaling, cancer | ||
| PRKACB | Apoptosis, MAPK, Wnt & Hedgehog signaling | ||||
| let-7i-5p | 12q14.1 | Up-reg’d | TLR4* | Toll-like receptor signaling, inflammatory response | |
| IL13* | Cell-cell signaling, Jak-STAT, Toll-like receptor signaling, cytokine-cytokine receptor interaction | ||||
| let-7i-3p | Up-reg’d | DLX5 | Cell proliferation | ||
| ACTB | Focal adhesion, leukocyte transendothelial migration | ||||
| mir-8/200 | miR-200a-5p | 1p36.33 | Up-reg’d | ZEB2 | EMT, Wnt signaling |
| FGF4 | Apoptosis, MAPK signaling | ||||
| miR-200a-3p | Up-reg’d | ZEB1* | EMT, transcriptional misregulation in cancer | ||
| ZEB2* | EMT, Wnt signaling | ||||
| miR-200b-5p | 1p36.33 | Down-reg’d | ZEB2 | EMT, Wnt signaling | |
| PRDM6 | Chromatin modification | ||||
| miR-200b-3p | Up-reg’d | ZEB1* | EMT, transcriptional misregulation in cancer | ||
| MLH1 | Mismatch repair, cancer | ||||
| miR-141-5p | 12p13.31 | Up-reg’d | ATM | Apoptosis, cell cycle, p53 signaling, cancer | |
| CLDN1 | Cell adhesion, leukocyte transendothelial migration | ||||
| miR-141-3p | Up-reg’d | MYC* | Angiogenesis, apoptosis, cancer, Jak-Stat, MAPK & Wnt signaling | ||
| TP53* | Angiogenesis, apoptosis, cell cycle, MAPK, p53 & Wnt signaling, cancer | ||||
| mir-17 | miR-17-5p | 13q31.3 | Up-reg’d | THBS1* | Angiogenesis, cell cycle, p53 signaling, cancer |
| E2F1* | Apoptosis, cell cycle, cancer | ||||
| miR-17-3p | Up-reg’d | CD44 | Angiogenesis, inflammation, cancer | ||
| MYB | Cancer | ||||
| miR-18a-5p | 13q31.3 | Up-reg’d | THBS1* | Angiogenesis, cell cycle, p53 signaling, cancer | |
| HIF1A* | Angiogenesis, cell migration, VEGF signaling | ||||
| miR-18a-3p | Up-reg’d | KRAS* | Apoptosis, MAPK & VEGF signaling, cancer | ||
| CASP7 | Apoptosis | ||||
| miR-20a-5p | 13q31.3 | Up-reg’d | THBS1* | Angiogenesis, cell cycle, p53 signaling, cancer | |
| E2F1* | Apoptosis, cell cycle, cancer | ||||
| miR-20a-3p | Up-reg’d | KRAS | Apoptosis, MAPK & VEGF signaling, cancer | ||
| PCNA | Cell cycle, cancer |
1A complete list of predicated/validated target transcripts and putative regulated biological functions is presented in Additional file 5. 2Asterisks in the column indicate validated targets as tabulated in miRBase. 3Data were derived from the Gene Ontology Annotation Database (UniProt-GOA) and the KEGG Pathway databases.