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. 2014 Oct 8;10:228. doi: 10.1186/s12917-014-0228-1

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© Jagarlamudi et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Standard curve for recombinant TK1 protein using the immune affinity assay and Western blot analysis. (A) Concentration curve obtained after scanning the relative band intensities (arbitrary units (AUs)). (B) The 28 kDa polypeptide bands of recombinant dog thymidine kinase 1 (TK1) (0.5–4 ng).