Table 1.
Pig | Cell culture | LC MS (NS/LPS/STM † ) | QRT PCR | FACS | Light microscopy |
---|---|---|---|---|---|
1 | A | Full FACS analysis* | Yes | ||
2 | A | Full FACS analysis | Yes | ||
3 | A | Full FACS analysis | Yes | ||
4 | A# | Yes | Yes | CD14 check | Yes |
B# | Yes | Yes | CD14 check | Yes | |
5 | A# | Yes | Yes | CD14 check | Yes |
B# | Yes | Yes | CD14 check | Yes | |
6 | A | Yes | CD14 check | Yes | |
7 | A | Yes | CD14 check | Yes | |
8 | A | Yes | CD14 check | Yes |
† NS non stimulated cells, STM S. Typhimurium.
*Full analysis comprises the FACS measurement of the following surface markers: CD11a, CD14, CD16, CD45, CD163, CD172α, MHC-II, TLR2, TLR4. FACS analysis was performed only for untreated and S. Typhimurium infected cell cultures.
#Two independent batches of MoMΦ and MoDC were obtained from these pigs.