Table 2.
Fold inductions of genes identified in this study in MoMΦ or MoDC in response to LPS or Salmonella Typhimurium (STM) exposure determined by QRT-PCR
| MoDC | MoDC fold induction $ | MoMΦ | MoMΦ fold induction | MoMΦ to MoDC | |||||
|---|---|---|---|---|---|---|---|---|---|
| Basal exp | STM | LPS | Basal exp | STM | LPS | NS | STM | LPS | |
| IL1B | 0.06 ± 0.04 | 106 # ± 101 | 2358 # ± 1268 | 0.11 ± 0.06 | 383 # ± 451 | 1999 # ± 1743 | n | * | n |
| AMCF2 | 0.40 ± 0.20 | 35.3 # ± 33.0 | 543 # ± 528 | 0.75 ± 0.52 | 115 # ± 107 | 305 # ± 267 | n | n | n |
| CXCL2 | 0.71 ± 0.28 | 17.4 # ± 19.1 | 200 # ± 161 | 0.19 ± 0.19 | 155 # ± 102 | 668 # ± 432 | n | n | n |
| IL1A | 0.28 ± 0.14 | 22.9 # ± 25.3 | 229 # ± 164 | 0.24 ± 0.12 | 86.5 # ± 48.8 | 187 # ± 57 | n | ** | n |
| IL8 | 15.3 ± 13.7 | 5.9 ± 5.74 | 34.2 # ± 21.6 | 6.78 ± 5.95 | 22.9 # ± 13.2 | 113 # ± 66 | n | n | n |
| ISG15 | 0.88 ± 0.52 | 2.94 # ± 1.22 | 2.00 ± 0.94 | 0.17 ± 0.10 | 22.1 # ± 2.0 | 78.9 # ± 104.4 | *** | n | n |
| MARCKSL1 | 0.70 ± 0.60 | 1.82 ± 0.63 | 4.07 # ± 2.30 | 0.25 ± 0.08 | 17.9 # ± 9.1 | 42.9 # ± 15.6 | n | * | ** |
| SOD2 | 4.28 ± 4.58 | 2.13 ± 0.80 | 12.8 # ± 12.9 | 4.61 ± 2.55 | 11.3 # ± 4.6 | 27.6 # ± 11.6 | n | ** | n |
| IκBα | 1.15 ± 0.36 | 2.74 # ± 1.12 | 8.12 # ± 3.10 | 1.13 ± 0.57 | 9.64 # ± 3.72 | 15.1 # ± 4.8 | n | *** | n |
| CD14 | 0.52 ± 0.19 | 3.84 # ± 1.52 | 14.4 # ± 9.6 | 0.35 ± 0.27 | 14.0 # ± 9.7 | 18.0 # ± 20.0 | n | n | n |
| PPA1 | 1.34 ± 0.84 | 1.85 # ± 0.7 | 4.83 # ± 1.93 | 1.39 ± 0.17 | 4.42 # ± 1.00 | 7.02 # ± 1.73 | n | *** | n |
| CD48 | 7.35 ± 4.25 | 1.83 ± 0.22 | 3.2 # ± 1.8 | 9.93 ± 2.43 | 3.34 # ± 0.71 | 5.33 # ± 1.90 | n | * | * |
| NF-κB1 | 1.57 ± 0.41 | 1.82 ± 0.61 | 3.49 # ± 1.4 | 1.97 ± 0.44 | 2.25 # ± 0.7 | 4.63 # ± 2.20 | n | n | n |
| ANXA1 | 15.3 ± 4.7 | 1.78 ± 0.9 | 3.74 # ± 1.91 | 22.9 ± 13.4 | 1.40 ± 0.38 | 2.45 # ± 1.01 | n | n | n |
| OPN | 86.1 ± 42.8 | 2.33 ± 1.5 | 3.42 # ± 2.27 | 138 ± 72 | 2.12 ± 1.20 | 3.81 # ± 2.00 | n | n | n |
| PFDN2 | 0.09 ± 0.03 | 1.54 ± 0.51 | 2.22 # ± 0.63 | 0.12 ± 0.04 | 2.12 # ± 0.60 | 2.63 # ± 0.72 | n | n | n |
| PSMB4 | 6.18 ± 1.58 | 1.42 ± 0.30 | 1.82 # ± 0.31 | 7.34 ± 1.39 | 1.73 ± 0.40 | 2.11 # ± 0.54 | n | n | n |
| MoDC only | |||||||||
| OLDLR1 | 9.73 ± 3.74 | 1.95 # ± 0.5 | 3.33 # ± 0.91 | 26.2 ± 3.0 | 1.43 ± 0.42 | 1.62 ± 0.41 | *** | * | n |
| CDC42 | 3.52 ± 0.70 | 1.62 # ± 0.33 | 1.63 ± 0.33 | 4.78 ± 1.43 | 1.60 ± 0.13 | 1.53 ± 0.61 | n | n | n |
| MoMΦ only | |||||||||
| VCAM1 | 0.33 ± 0.25 | 2.32 ± 1.90 | 5.52 ± 4.51 | 0.18 ± 0.19 | 9.82 # ± 6.24 | 18.9 # ± 13.6 | n | n | n |
| HMOX1 | 0.32 ± 0.20 | 2.32 ± 1.53 | 1.42 ± 0.51 | 0.45 ± 0.11 | 2.34 # ± 1.11 | 2.44 # ± 0.14 | n | n | * |
| Serglycin | 45.9 ± 19.9 | 1.52 ± 0.71 | 2.40 ± 1.03 | 35.7 ± 28.9 | 3.04 # ± 1.32 | 2.84 # ± 1.33 | n | n | n |
$The fold inductions are presented as mean ± SD calculated based on ratios of individual treated samples relative to the mean of appropriate non-stimulated control.
#The numbers signed with #represent significantly different results compared to non-stimulated (NS) cells.
*Statistically significant differences between MoMΦ and MoDC with the same treatment. *p < 0.05; **p < 0.01; ***p < 0.001; n = non-significant.