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. 2014 Oct 9;14:101. doi: 10.1186/s12935-014-0101-0

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© Hoe et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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SP subpopulation enriches for cells expressing stem-cell related genes. Combined data from Stem Cell PCR Array and Stem Cell Signaling PCR Array were clustered according to gene function or signaling pathways. Gene expression data were normalized to five housekeeping genes via geometric mean calculation. Only genes which were significantly upregulated by at least 2 fold, with raw Ct values of less than 35 are shown here. None of the downregulated genes fulfilled the selection criteria of at least 2 fold change and p < 0.05. Some of the genes appeared in more than 1 gene function or signaling pathway (refer to Additional file 2 for details).