Table 6.
Expression levels of the chitin synthesis related genes
| RNA-seq ID | L5D6-RPKM | PP-RPKM | P0-RPKM | Gene description |
|---|---|---|---|---|
| Bm_nscaf2800_23 | 20.24 | 12.65 | 8.76 | trehalase-2 |
| Bm_nscaf2800_24 | 11.56 | 9.11 | 13.95 | trehalase-2 |
| Bm_nscaf2829_133 | 0.21 | none | 1.28 | trehalase precursor |
| Bm_nscaf2829_134 | 0.42 | 1.88 | none | trehalase 1B |
| Bm_nscaf3027_062 | 6.90 | 15.30 | 8.68 | hexokinase type 2-like isoform X3 |
| Bm_nscaf3027_063 | 47.91 | 105.58 | 100.81 | hexokinase type 2-like isoform X3 |
| Bm_nscaf2780_10 | 122.98 | 145.82 | 36.81 | glucose-6-phosphate isomerase |
| Bm_nscaf2887_059 | 53.54 | 51.33 | 675.08 | glutamine:fructose-6-phosphate aminotransferase |
| Bm_nscaf2823_132 | 77.53 | 155.12 | 886.62 | glucosamine-6-phosphate N-acetyltransferase |
| Bm_nscaf3003_158 | 13.61 | 56.53 | 34.73 | phosphoacetylglucosamine mutase |
| Bm_nscaf2176_247 | 17.11 | 14.13 | 436.84 | UDP-N-acetylhexosamine pyrophosphorylase |
| Bm_nscaf2589_266 | 49.30 | 138.56 | 277.03 | chitin synthase A |
| Bm_nscaf2589_269 | 3.55 | 0.27 | none | chitin synthase B |
| Bm_nscaf2589_270 | 5.42 | none | none | chitin synthase B |
The gene expression level was calculated by RPKM method. "none" means that the expression of the gene was not detected. The genes with black bold ID were selected for qRT-PCR analysis.