Table 4. UV melting points and hyperchromicities of dodecamer duplexes.

Probe	Target sequence	Tma (°C)		ΔTm 1 Mb (°C)	Hyperchromicityc (%)
		[salt] = 100 mM	[salt] = 1 M
AATTTCTTTCTT	AAGAAAGAAATT	38.4 ± 0.3	42.1 ± 0.6	–	30.9 ± 0.2
19	AAGAAAGAAATT	48.4 ± 0.6	53.2 ± 0.5	+11.1	22.6 ± 0.2
TTTTTCTTTCTT	AAGAAAGAAAAA	35.6 ± 0.9	37.3 ± 0.9	–	30.4 ± 0.5
20	AAGAAAGAAAAA	47.7 ± 0.3	52.9 ± 0.3	+15.6	29.3 ± 1.2
24	AAGAAAGAAAAA	45.0 ± 0.9	46.3 ± 0.9	+9.0	26.1 ± 1.1
TTTTTCTTTCTT	CGAAAGAAAGAAAAAAGC	38.2 ± 0.2	42.1 ± 0.4	–	26.2 ± 0.8
20	CGAAAGAAAGAAAAAAGC	48.7 ± 0.3	53.7 ± 0.4	+11.6	30.9 ± 1.5

^aAverage of four melting points ± SD at 1.6 µM strand concentration and 10 mM PIPES buffer, 10 mM MgCl₂ and NaCl concentration given.

^bMelting point difference to fully complementary duplex.

^cHyperchromicity upon duplex dissociation at 260 nm.