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. 2004 May 25;32(9):2966–2974. doi: 10.1093/nar/gkh601

Figure 2.

Figure 2

(A–D) Kinetics of the HPR-catalyzed ligation in trans at different temperatures (shown at the right). (A) Ligation of equimolar amounts of the 3′-LS and 5′-LS, at HPR-to-substrate ratio 1:1, 5 min to 24 h; (B) the enlarged initial 5–60 min time interval for (A). (C) Ligation of equimolar amounts of 3′-LS and 5′-LS, at HPR-to-substrate ratio 20:1, 5 min to 24 h; (D) the enlarged initial 5–60 min time interval for (C). Average values from three experiments are shown. (E–F) Initial rates (E) and ligation yields (F) for HPR-catalyzed ligation in trans at different temperatures, based on data presented in (A–D). HPR-to-substrate ratios 20:1 (triangles) or 1:1 (squares).