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HPR derivatives with ligation yields observed after 14 h incubation at –10 and 37°C. Ligation substrates 3′-LS and 5′-LS were used in equimolar amounts, and the ribozyme-to-substrate ratio was 20:1. I, HPR; II, bisected HPR; III, truncated HPR; IV and V, HPR with separated domains; VI, template-deleted HPR.
