Figure 6.

CsdA associates with the 40S ribosomal particle from the ΔsrmB extract. (Top) Sedimentation profile showing the free subunits from ΔsrmB cells carrying a FLAG-tagged csdA gene (WJW45ΔsrmB FLAG-csdA). Cells were grown at 20°C. (Bottom) Equal volumes of each fraction were subjected to western blotting using anti-FLAG, anti-L1 and anti-S3 antibodies. Total proteins isolated from WJW45ΔsrmB (T1) and WJW45-FLAG-csdA (T2) strains were used as negative and positive controls for FLAG detection, respectively.