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. 2014 Oct 9;7:151. doi: 10.1186/s13068-014-0151-5

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© Inoue et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Synergistic hydrolysis of PCS by the purified enzyme mixture. Hydrolysis was carried out at pH 5, 45°C, with 3% (w/v) pretreated corn stover (PCS) and a protein loading of 2.55 mg/g glucan, consisting of 40 μg of the mixed cellulolytic enzymes and 5 μg of Bgl3A in a final volume of 1 mL. The proportions (%) of the mixed cellulolytic enzymes are noted under the columns. The percent hydrolysis of PCS glucan was calculated from the amount of glucose in the hydrolysate after 48 hours. 5A, Cel5A; 6A, Cel6A; 7A, Cel7A; 7B, Cel7B; 10A, Xyl10A.