Figure 3.

DNA sequencing. (A) The same sequencing reagents with polymerase (P) and without it (N) were fed into same-device chambers at different times. Tagged nucleotide incorporation was confirmed by the P-N difference in each case. P’s over time show sequencing reagents retain activity over at least 2 h. (B) A mixture of tagged and non-tagged nucleotides of the same type is fed into a single sequencing chamber. Incorporation is detected as increase in fluorophore surface density. The read GAUG corresponds exactly to the template sequence CTAC. (C) Statistics over nine sequencing experiments shows the most likely read length is 3 bp from a single chamber.