Figure 1. Comparison of CTC enumeration performed by SET-iFISH and CellSearch.

(A) Images of CTCs revealed by SET-iFISH and CellSearch. CTCs identified by SET-iFISH are DAPI⁺ (blue)/FISH⁺ (aneuploid chromosome 8, orange)/CKs^{+ or –} (green) and CD45⁻; CTC identified by CellSearch are CKs⁺/DAPI⁺/CD45⁻. Scale bar = 5 μm. (B) Enumeration of CTCs by SET-iFISH and CellSearch in healthy donors (Control), AGC patients before and after treatment.