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. 2014 May 8;5(16):6633–6646. doi: 10.18632/oncotarget.1955

Figure 5. ADAM10 and betacellulin levels are increased in acquired trastuzumab resistant cells compared to naïve cells.

Figure 5

Resistant cells were continuously treated with 40μg/ml trastuzumab. (A) ADAM10 mRNA and protein levels of naïve and acquired trastuzumab resistant BT474 and SKBR3 cells were assessed. A representative blot and the semi-quantification of three blots are shown. (B) The naïve and acquired trastuzumab resistant BT474 and SKBR3 cells were seeded and the media was replaced the next day by serum-free media for 24h before ELISA. (C) ADAM10 was knocked down in resistant SKBR3 cells and the level of betacellulin in the media was measured by ELISA. Graphs show means ± SD.