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. 2014 Jul 13;5(16):6670–6686. doi: 10.18632/oncotarget.2213

Figure 8. Effect of PFKFB3 or PFKFB4 siRNA Transfection on Apoptosis.

Figure 8

H460 cells were transfected with control siRNA (siCtrl), PFKFB3 siRNA (siFB3) or PFKFB4 siRNA (siFB4), cultured in 21% oxygen or 1% oxygen for 48 hours, and analysed for apoptosis by flow cytometry of propidium iodide and annexin V positive cells (A and B, PI+ + PI/Ann V+ cells shown as % apoptotic cells) and by cleaved PARP by Western blot analysis and densitometry (C). Data are expressed as the mean ± SD of three experiments. * p value < 0.005 compared to control siRNA.