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. 2014 Jul 25;5(16):6801–6815. doi: 10.18632/oncotarget.2254

Figure 5. AZD1480 and ruxolitinib do not affect proliferation of murine T cells but negatively impact on their IFN-γ secretion in vitro.

Figure 5

Splenocytes of naive mice were treated with different concentrations of AZD1480, sunitinib, axitinib or ruxolitinib and after three days, proliferation of CD4+ and CD8+ T cells was determined by flow cytometry. Controls included T cells with and without T-cell stimulation. A. Representative FACS profile of the proliferation of CD8+ and CD4+ T cells in the presence of different concentrations of AZD1480. B. Overview of the proliferation of CD8+ and CD4+ T cells in the presence of different concentrations of AZD1480. Five independent experiments were performed and results are presented as mean ± SEM. IFN-γ secretion by splenocytes was determined after three days of culture in the presence of different concentrations of AZD1480. Five independent experiments were performed and results are presented as mean ± SEM. C. Overview of the proliferation of CD8+ and CD4+ T cells in the presence of different concentrations of sunitinib. Three independent experiments were performed and results are presented as mean ± SEM. IFN-γ secretion by splenocytes was determined after three days of culture in the presence of different concentrations of sunitinib. Three independent experiments were performed and results are presented as mean ± SEM. D. Overview of the proliferation of CD8+ and CD4+ T cells in the presence of different concentrations of axitinib. Three independent experiments were performed and results are presented as mean ± SEM. IFN-γ secretion by splenocytes was determined after three days of culture in the presence of different concentrations of axitinib. Three independent experiments were performed and results are presented as mean ± SEM. E. Overview of the proliferation of CD8+ and CD4+ T cells in the presence of different concentrations of ruxolitinib. Three independent experiments were performed and results are presented as mean ± SEM. IFN-γ secretion by splenocytes was determined after three days of culture in the presence of different concentrations of ruxolitinib. Three independent experiments were performed and results are presented as mean ± SEM.