Figure 6. CD14HLA-DR MDSCs suppress the proliferation of CD8 T cells but AZD1480 treatment does not affect their suppressive activity.

Both CD14⁺HLA-DR^low myeloid-derived suppressor cells and CD14⁺HLA-DR^high monocytes were sorted from peripheral blood mononuclear cells and co-cultured for 6 days with autologous CD8⁺ T cells in the presence of anti-CD3/CD28 beads. Controls included T cells cultured in the absence of MDSCs with and without T-cell stimulation. A. Overview of the percentage of proliferating CD8⁺ T cells cultured in the presence of different ratios of myeloid-derived suppressor cells (left panel) and monocytes (right panel). B. Different concentrations of AZD1480 were added to co-cultures of myeloid-derived suppressor cells and CD8⁺ T cells and after 6 days proliferation of T cells was determined. The percentage of proliferating CD8⁺ T cells (left panel) and the percentage of suppression (right panel) are shown.