Skip to main content
. 2014 Jul 17;5(16):6896–6908. doi: 10.18632/oncotarget.2229

Figure 3. dASOs 6w2 and 6w5 induce apoptosis, cell cycle arrest and abolish NFkB signaling.

Figure 3

(A-B) Annexin V assay of PC-3 cells treated with dASOs 6w2 and 6w5 for 72 hr. (A) FACS plot showing cells under early apoptosis as identified by Annexin V +, propidium iodide (PI) -. (B) Mean percentage of early apoptotic cells from Annexin V assay. Error bars represent mean ± S.D. (C-D) DAPI staining of dASO-treated cells. (C) Representative images of PC-3 cells stained with DAPI after 72 hr of dASO treatment; apoptotic cells were identified by fragmented nuclei. (D) Quantification of cells undergoing apoptosis: percentage of fragmented nuclei. (E) Cell cycle distribution of PC-3 cells treated with ASOs for 72 hr as determined by PI staining. (F) Percentage of cells at the G2-M phase. (G) NFkB transcription activation was examined using a NFkB dual luciferase reporter assay. PC-3 cells were co-transfected with dASOs, NFkB-responsive firefly luciferase and Renilla luciferase plasmid. Luciferase activity was measured at 48 hr after transfection with prior induction by TNF-α treatment.