Figure 5. Deficiency of tuberin results in increased vimentin protein expression in tumor kidney tissue of TSC patients.

(A) Representative Immunoblot analysis showed significant decreased in tuberin and increased in vimentin protein expression in tumor kidney (T) from patients with tuberous sclerosis compared to normal kidney tissues. Actin was used as loading control. (B) Histograms represent means±SE (n=6). Significant difference from control is indicated by **P<0.01. (C) H&E staining shows (a) a normal tubular and glomerular structure in control kidney tissue and (b) *fat, ^vessel and # smooth muscle cells types in kidney angiomyolipoma tissue of TSC patients. (D) Kidney sections were stained with vimentin followed by immunofluroscene staining. Control of kidney (c) shows a few cells stained with vimentin while (d) most of blood vessel and smooth muscle cells were stained in kidney tumor tissue. Control sections in both procedures were incubated without primary antibody.