Fig.2. scFv78 fusion proteins demonstrate higher avidity to cell-surface TEM1.
A, The dose-dependent binding curves of scFv78 and its derivatives. Cell-surface binding activity in control (MS1) or TEM1-expressing cells (MS1-TEM1) was measured by a live cell-based ELISA assay. TEM1-specific binding was calculated as the difference between binding to the control and TEM1-expressing protein. The control anti-TEM1 IgG is a biotinylated humanized mouse IgG1. B, A summary of the calculated Kd and Bmax of the scFv78 and scFv78 derivatives. Anti-HA HRP was used as the secondary antibody in ELISA for all proteins except for the control anti-TEM1 IgG which required strepavidin-HRP. * indicates the different secondary antibody used.