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. 2014 Jun 1;5(16):7027–7039. doi: 10.18632/oncotarget.2053

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Copyright: © 2014 Boesch et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Defined mixtures of corresponding SP and NSP fractions were stained for different markers (staining 1 of Suppl. Table 4) and analyzed by multicolor flow cytometry. (A) Definition of populations positive for individual markers (cell line: A2780). These gates were combined by Boolean operations to obtain proportions of cells within all possible combinations, which were then imported into SPICE for final data analysis. (B) SPICE analysis of ovarian cancer heterogeneity after class-division into SP (upper panel) and NSP (lower panel). Subset distributions are presented in the weighted category mode, and only subsets above a threshold of ≥0.1% are shown. Data are representative examples of at least two independent experiments. SP, side population; NSP, non-SP.