Figure 2. Ectopic expression of XIAP^ΔRING in XIAP−/− cells resulted in upregulation of Cyclin E protein expression.

(A) stable HCT116 XIAP−/− cell transfectants used in our study were identified by Western blot. (B & C) the cells were synchronized by incubation of cells with 0.1% FBS medium for 24h. The cells were then cultured in 2% FBS medium for 24 h and cell extracts were subjected to Western blot for determination of protein expression as indicated (B), and quantified (C).