Figure 3. Cyclin E induction was essential for XIAP^ΔRING promotion of cancer cell anchorage-independent growth and G₁/S phase cell cycle transition.

(A) immunoblot analysis of Cyclin E protein levels in XIAP−/−(HA-XIAP^ΔRING) cells transfected with Cyclin E shRNA. (B) after synchronization of cells with 0.1% FBS medium, cells were cultured in 2% FBS medium for 24 h, and cells were then subjected to flow cytometry analysis. (C & D) stable transfectants as indicated were subjected to anchorage-independent growth assay in soft agar. The symbol (*) indicates a significant inhibition of colony formation by knockdown of Cyclin E expression (p<0.05). The colonies were expressed as mean±S.D.