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. 2014 Jul 31;5(16):7183–7197. doi: 10.18632/oncotarget.2293

Fig.1. Overexpression of Apollon and downregulation of Smac in ESCC cell lines and clinical ESCC tissues.

Fig.1

(A) Protein levels of Apollon and Smac in normal human esophageal epithelial cells (NEEC) and cultured ESCC cell lines were analyzed by Western blotting. Apollon (B) and Smac (C) protein levels were quantificated in NEEC and cultured ESCC cell lines. Protein levels were normalized for GAPDH. Data are represented as Means ± standard deviations (SD) from three independent experiments. *P < 0.05 and **P < 0.01 vs. NEEC. (D) Typical patterns of Apollon expression analyzed by Western blotting in paired ESCC tissue samples. N, adjacent non-tumorous tissues; T, tumor tissues. Quantification of Apollon (E) and Smac (F) protein in 30 paired ESCC tissue samples. Protein levels were normalized for GAPDH. **P < 0.01.