. 2004 May 20;32(9):e75. doi: 10.1093/nar/gnh067

Table 2. Molecular weight (M_w), corrected response, interaction stoichiometry (hybridization efficiency for probe/target interactions), interaction rate constants k_on and k_off and affinity constant K_A for the interactions shown in Figures 2 and 3.

Name	M_W (kDa)	Response^a	Stoichiometry^b	k_off (s^–1)	k_on (M^–1 s^–1)	K_A (M^–1)
Streptavidin	∼60	5.1	0.75
Biotinylated probe	9.488	1.0
T15-MM0	4.622	0.42	0.84	1.32 × 10^–4	6.58 × 10⁴	4.98 × 10⁸
T15-MM1	4.607	0.31	0.62	1.11 × 10^–3	2.42 × 10⁴	2.18 × 10⁷
T75-MM0	22.873	1.14	0.46	3.52 × 10^–5	9.21 × 10³	2.62 × 10⁸
T75-MM1	22.858	0.67	0.27	7.41 × 10^–4	1.08 × 10⁴	1.46 × 10⁷

^aDefined as the increment in corrected diffraction intensity (see text for details) normalized to the response of the biotinylated probe.

^bDefined as stoichiometry = (streptavidin or DNA target response × M_w of biotinylated probe)/(biotinylated probe response × M_w of streptavidin or DNA target).

Table 2. Molecular weight (Mw), corrected response, interaction stoichiometry (hybridization efficiency for probe/target interactions), interaction rate constants kon and koff and affinity constant KA for the interactions shown in Figures 2 and 3.

Table 2. Molecular weight (M_w), corrected response, interaction stoichiometry (hybridization efficiency for probe/target interactions), interaction rate constants k_on and k_off and affinity constant K_A for the interactions shown in Figures 2 and 3.