Table 2. Molecular weight (Mw), corrected response, interaction stoichiometry (hybridization efficiency for probe/target interactions), interaction rate constants kon and koff and affinity constant KA for the interactions shown in Figures 2 and 3.
Name | MW (kDa) | Responsea | Stoichiometryb | koff (s–1) | kon (M–1 s–1) | KA (M–1) |
---|---|---|---|---|---|---|
Streptavidin | ∼60 | 5.1 | 0.75 | |||
Biotinylated probe | 9.488 | 1.0 | ||||
T15-MM0 | 4.622 | 0.42 | 0.84 | 1.32 × 10–4 | 6.58 × 104 | 4.98 × 108 |
T15-MM1 | 4.607 | 0.31 | 0.62 | 1.11 × 10–3 | 2.42 × 104 | 2.18 × 107 |
T75-MM0 | 22.873 | 1.14 | 0.46 | 3.52 × 10–5 | 9.21 × 103 | 2.62 × 108 |
T75-MM1 | 22.858 | 0.67 | 0.27 | 7.41 × 10–4 | 1.08 × 104 | 1.46 × 107 |
aDefined as the increment in corrected diffraction intensity (see text for details) normalized to the response of the biotinylated probe.
bDefined as stoichiometry = (streptavidin or DNA target response × Mw of biotinylated probe)/(biotinylated probe response × Mw of streptavidin or DNA target).