FIG 2 .

H-NS and SlyA influence the levels and timing at which mRNAs for PhoP-activated genes are produced. mRNA levels for PhoP-dependent genes after bacteria were shifted from LB broth containing 10 mM Mg²⁺ to LB broth. We investigated the H-NS and SlyA-independent phoP gene, the H-NS-dependent and SlyA-independent pcgL gene, and the virK, mig-14, and pagC genes, which are H-NS- and SlyA-dependent. mRNA in the rpoS strain (MJC140), rpoS hns strain (MJC138), rpoS hns slyA strain (MJC139), and rpoS slyA strain (MJC141) displaying four different expression behaviors in terms of timing and levels at which the mRNAs are produced: early and high (phoP) (A), middle and low (pcgL) (B), late and high (virK) (C), and late and low (pagK) (D). (E to H) H-NS and SlyA influence the timing at which mRNAs for PhoP-activated genes are produced. Normalized mRNA levels corresponding to panels A to D displaying differential onset times.