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Signal generation is entirely dependent upon transcription from an added DNA template when a molecular beacon possessing a 2′-O-methylribonucleotide backbone [MB-poly(A)-FAM-2′-O-methyl] is used as the probe. The fluorescence of a reaction initiated with a DNA template [T7-sinR-poly(A)] (continuous line) was compared with the fluorescence of an otherwise identical template-free reaction (dotted line).
