Table 1. DNA templates (coding strands) and molecular beacons used in transcription reactions.

Templates
	T7 promoter sinR poly(A)
T7-sinR-poly(A)	5′-GACTAATACGACTCACTATAGGGAGGA|TTTGCTCGATGAGAAA……….AAACAATTTCGTGAATTT|(A)25-3′	167 bp
	T3 promoter sinR poly(A)
T3-sinR-poly(A)	5′-GACTTATTAACCCTCACTAAAGGGAAG|TTTGCTCGATGAGAAA……….AAACAATTTCGTGAATTT|(A)25-3′	167 bp
	T7 promoter rpoB poly(A)
T7-rpoB-poly(A)	5′-GACTAATACGACTCACTATAGGGAGGA|GGCCGGTGGTCGCCGCG……….GCCCGGCGGTCTGTCACGT|(A)25-3′	187 bp
	T7 promoter sinR
T7-sinR	5′-GACTAATACGACTCACTATAGGGAGGA|TTTGCTCGATGAGAAA……….AAACAATTTCGTGAATTT-3′	161 bp
	sinP3 promoter sinR poly(A)
sinP3-sinR-poly(A)	5′-GAAGTCATACCGTAAATCCTTTCTGAATGTGCTATAATATCACAAGG|TTTGCTCGATGAGAAA……….AAACAATTTCGTGAATTT|(A)25-3′	187 bp
Molecular beacons
MB-sinR-TET-2′-O-methyl	5′-tetrachlorofluorescein-CGCU UUUCGAUACCCCGGAUGUCAU AGCG-dabcyl-3′
MB-poly(A)-FAM-2′-O-methyl	5′-fluorescein-CGC UUUUUUUUUUUU GCG-dabcyl-3′
MB-poly(A)-FAM-DNA	5′-fluorescein-CGCTC TTTTTTTTTTTTTTTTTTTT GAGCG-dabcyl-3′