TABLE 2 .
Transposition frequencies of DDE mutants
| Plasmid | IS26 | Target | Transposition frequencya |
||
|---|---|---|---|---|---|
| Mean | No.b | Range | |||
| pRMH977 | IS26 | R388 | 1.7 × 10−7 | 3 | 5.1 × 10−8–3.7 × 10−7 |
| pRMH978 | IS26-FS-Lc | R388 | <1.8 × 10−8 | 3 | <1.1 × 10−8–<1.9 × 10−8 |
| pRMH987 | IS26 D82L | R388 | <5.3 × 10−8 | 3 | <4.3 × 10−8–<7.1 × 10−8 |
| pRMH988 | IS26 D142L | R388 | <3.4 × 10−9 | 3 | <2.8 × 10−9–<4.2 × 10−9 |
| pRMH989 | IS26 E177L | R388 | <4.4 × 10−8 | 3 | <1.8 × 10−8–<5.9 × 10−8 |
| pRMH977 | IS26 | R388::IS26 | 2.1 × 10−4 | 3 | 1.9 × 10−4–2.4 × 10−4 |
| pRMH978 | IS26-FS-Lc | R388::IS26 | 8.4 × 10−6 | 3 | 7.3 × 10−6–9.2 × 10−6 |
| pRMH987 | IS26 D82L | R388::IS26 | 6.4 × 10−6 | 3 | 4.8 × 10−6–9.6 × 10−6 |
| pRMH988 | IS26 D142L | R388::IS26 | 6.4 × 10−6 | 3 | 3.5 × 10−6–1.1 × 10−5 |
| pRMH989 | IS26 E177L | R388::IS26 | 9.7 × 10−6 | 3 | 4.3 × 10−6–2.0 × 10−5 |
Transposition frequency expressed as cointegrates per transconjugant.
Number of independent determinations.
IS26-FS-L is an IS26 tnp26 frameshift mutant.