FIG 5 .

Latrunculin A-induced actin rods bind the major capsid protein. MEF cells constitutively expressing Lifeact-EGFP were infected with PRV mRFP-VP26. Cells at 4 hpi were either mock treated (mock) or incubated for 1 h with 4.7 µM (2 µg/ml) LatA (LatA). Cells were lysed, and the precleared lysates were incubated with antibodies directed against glutathione S-transferase (GST) or GFP, detecting Lifeact-EGFP (LA-GFP) and protein A/G beads for 1.5 h at 4°C. Bead-bound immunoprecipitates were separated from supernatant and washed extensively. Ten percent of lysate (input) and supernatants (sup) and 50% of immunoprecipitated protein (pellet) were subjected to SDS-PAGE and Western blotting using antiactin, anti-GFP, and anti-VP5 antibodies.