Fig. 3.

Identification and characterization of the tRNA charged by YadB. (A) PAGE in 40 mM Mops (pH 6.5)/10 mM sodium acetate/1 mM EDTA. Lane 1, 2 μg of purified RNA charged with l-[¹⁴C]Glu by E. coli YadB; lanes 2–4, 40 μgof unfractionated E. coli tRNA charged with l-[¹⁴C]Glu by E. coli YadB or GluRS or with l-[¹⁴C]Gln by GlnRS, respectively. The RNAs were stained with methylene blue. (B) PhosphorImager exposure of the gel shown in A. (C) Homochromatogram of RNA fragment specific of E. coli tRNA^Asp and cloverleaf fold of this tRNA. The oligonucleotide identifying tRNA^Asp is displayed on a black background.