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. 2004 Apr 19;101(20):7530–7535. doi: 10.1073/pnas.0401634101

Fig. 4.

Fig. 4.

In vivo expression of E. coli YadB gene. (A) Physical map of the E. coli dksA-yadB region showing the localization of the RT-PCR amplification targets. The promoter for dksA identified by Kang and Craig (47) is indicated by the letter P. (B) Expression of the YadB mRNA in E. coli K12 grown in LB medium/agarose gel electrophoresis of the products of the RT-PCR reactions and of controls without the reverse polymerase (R.T.). (C) Expression of the YadB protein. Lanes 1–3, analysis by Western blot of, respectively, 10 ng of overexpressed pure YadB and 9 μg of crude and S100 extracts from wild-type E. coli.