. 2004 Apr 19;101(20):7530–7535. doi: 10.1073/pnas.0401634101

Table 2. Kinetic constants of glutamylation and aspartylation of E. coli tRNA^Asp by YadB and AspRS.

	E. coli tRNA^Asp
	K_m, μM				k_cat/K_m, μM·s^-1
Enzyme	tRNA	ATP	Amino acid	K_cat, s^-1	tRNA	Amino acid
YadB	0.15	170	3,000	2.2	14.7	0.73 × 10^-3
AspRS	0.2	ND	22	6.8	34	0.31
k_cat/K_m[AspRS]/k_cat/K_m[YadB] (tRNA)					2.31
k_cat/K_m[AspRS]/k_cat/K_m[YadB] (amino acid)					424

The conditions are described in Materials and Methods.

Table 2. Kinetic constants of glutamylation and aspartylation of E. coli tRNAAsp by YadB and AspRS.