Fig. 3.

Analysis of periodate-treated Glu–tRNA^Asp and Asp–tRNA^Asp. (A) Glu–tRNA^Asp and Asp–tRNA^Asp was treated with periodate and then lysine, deacylated, and subjected to gel electrophoresis for size determination. tRNA was visualized by hybridization with ³²P-labeled probes against tRNA^Asp. N, position of intact tRNA^Asp; N-1, tRNA^Asp without the terminal adenosine; N^*, YadB-generated Glu–tRNA^Asp lacking the terminal adenosine; tRNA control. (B) Glu–tRNA^Asp was treated with periodate, deacylated and then recharged with either glutamate and YadB (○) or aspartate and E. coli AspRS (•). Untreated tRNA is aminoacylated by YadB (□) and AspRS (▪).