Table 1. Oligonucleotide primers used for PCR.
Name | Sequence | Forward/reverse | Corresponding amino acid sequence |
---|---|---|---|
KLK-His | 5′-GGT(GT)(ACTG)TCAC(AG)GC(CT)GC(CT)CA(CT)TG-3′ | Forward | WV(LV)TAAHC |
KLK-Ser | 5′-CCTGA(AG)TCTCCC(ACTG)(CT)ACA(AG)GTGTC-3′ | Reverse | D(ST)CVGDSG |
Oligo(dT) | 5′-CGCAGGAATTTTTTTTTTTTTTT-3′ | Reverse | |
KLK-1 | 5′-GGCAGGACCAGTCAGAACTATG-3′ | Forward | GRTSQNY |
KLK-2 | 5′-CCCATGCCCACAAGTTTAAGG-3′ | Forward | HAHMFK |
KLK-3 | 5′-GAAGGTCTTGTAGAAGAACGTCAG-3′ | Reverse | LTFFYKTF |
KLK-4 | 5′-CTATCACCATGTGTTTCCTGCTCC-3′ | Forward | MCFLL |
KLK-5 | 5′-GGGTGTTTAGCTGTGTGCCTTGA-3′ | Reverse | IKAHS* |
The terminal codon