Fig. 5.

INCA-6 inhibits the induction of NFAT-dependent cytokine mRNAs in Cl.7W2 T cells. (a) RNase protection assay for transcripts of the genes encoding TNF-α, TNF-β, and IFN-γ, and of the housekeeping genes L32 and GAPDH. Cells were preincubated with INCA-6 or a combination of CsA and FK506 where indicated, and further stimulated with PMA or a combination of PMA and ionomycin. (b and c) Quantitation of radiolabel in protected probes for TNF-α and IFN-γ mRNAs, presented in the same order as the lanes in a. -, unstimulated; P, stimulated with PMA alone; PI, stimulated with PMA and ionomycin.