Fig. 2.

Effect of a nefopam pretreatment on the withdrawal response to mechanical and thermal stimuli in streptozotocin (STZ)-induced diabetic rats. Pretreatment with nefopam (30 mg/kg) was given intraperitoneally 30 min prior to an intraperitoneal injection of STZ (60 mg/kg). Behavioral tests were performed before, and 1 to 4 weeks after the final administration of drugs. Systemic pretreatment with 30 mg/kg of nefopam significantly inhibited STZ-induced mechanical allodynia at 4 weeks (A). Pretreatment with nefopam significantly inhibited STZ-induced cold allodynia from 2 weeks after the nefopam treatment (B). However, a nefopam pretreatment did not attenuate STZ-induced thermal hyperalgesia (C). The data are presented as the means ± standard error. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001 compared to the control group. ^†P < 0.05 and ^††P < 0.01 compared to the STZ group.