Figure 5.

γH2AX staining of the islets in non-diabetic and diabetic subjects with or without amyloid deposition. (A) Immunoreactions for γH2AX were identified as positive control of nuclear reactions in apoptotic cells in the lymphnode (LN). The population of γH2AX positive cells was the largest in amyloid-rich diabetic group (DA + ) but not so marked in amyloid-free diabetic group (DA−). (B) Comparison of the frequency showed a significant increase in γH2AX-positive cells in DA+ compared to ND and DA− (*p < 0.01 versus ND and DA−). Population of γH2AX-positive cells was inversely correlated with β-cell volume density (p < 0.05) but not with amyloid volume density in DA+.