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. 2014 Apr 10;28(1-2):53–69. doi: 10.3109/01677063.2014.882918

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© 2014 Informa Healthcare USA, Inc.

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Figure 1. — Identification of pyramidal neurons and LCM procedure. Photomicrograph of a pyramidal neuron in the STG visualized with Histogene stain (A). After identification (B), pyramidal neurons are captured (C) onto the LCM cap (D). (E) Representative virtual gels showing the distribution of sizes of amplified products after two rounds of linear amplification of RNA extracted from ˜500 pyramidal neurons from a normal control (C) and a schizophrenia (S) subject. L = ladder. (F) Heatmap of 1331 differentially expressed genes identified based on the stringency criteria of fold-change > 1.1 and FDR-corrected p < 0.05. Scale bars = 25 μm.